OBJECTIVE: In recent years, some evidence has been presented among control angiotensin converting enzyme (ACE), methylenetetrahydrofolate reductase (MTHFR) gene mutation, and migraine. The aim of this study is to compare ACE and MTHFR with the migraine control group and to understand the relationship between them. STUDY DESIGN: This study was conducted between September 2012 and January 2013 at the Department of Neurology, Dicle University, Faculty of Medicine. This study consisted of 219 individuals, 119 (mean age 39.4±10.8) of whom were migraine patients and the remaining 100 (mean age 35.5±11.6) of whom made up the control group. In the evaluation of ACE genotype, polymerase chain reaction was determined using real-time PCR technique. RESULTS: The frequency of DD, ID, and II genotypes in the patient group was 33.0%, 61.5%, and 5.2%, respectively, while it was 53.6%, 43.6%, and 2.6% in the control group. It has been statistically shown that the ID genotype in the patient group was highly meaningful as compared to that in the control group. No risk difference has been witnessed both with aura and without aura in the migraine group as compared to the ACE gene DD, ID, and II polymorphism and MTHFR mutations. CONCLUSION: ID genotype was observed frequently in migraine (with and without aura). When the ACE gene was compared with DD, ID, and II polymorphisms and MTHFR mutations, no correlation was detected with or without aura. In our population, no significant increase in the D allele in the ACE gene and MTHFR T mutation was detected. © Science Printers and Publishers, Inc.